Tag

Part:BBa_K4161505

Designed by: Lingrui Lai   Group: iGEM22_DKU   (2022-07-26)


10aa T7 tag, 8aa Xpress Tag

T7 and Xpress tags can be used both for detection and purification of the target protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 79
    Illegal PstI site found at 84
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 79
    Illegal NheI site found at 1
    Illegal PstI site found at 84
    Illegal NotI site found at 105
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 79
    Illegal BamHI site found at 64
    Illegal XhoI site found at 112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 79
    Illegal PstI site found at 84
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 79
    Illegal PstI site found at 84
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None